2024-2025 Grant in Aid of Research
Expression of RASGRP1 in Bipolar Derived Lymphoblastoid cell lines.
Serena Sarsah
Bipolar disorder (BD) serves as a significant public health challenge. It is characterized by recurrent episodes of mania and depression. BD is broadly categorized into two subtypes Bipolar I (BD I) and Bipolar II (BD II). BD’s complex etiology involves genetic predisposition and environmental factors, however, the understanding of the molecular mechanisms underlying BD remains incomplete. Notably, family history is a significant risk factor in BD (McGuffin et al., 2003). Recent breakthroughs in genetic studies have identified RASGRP1, a gene involved in immune function and inflammatory responses as a candidate gene which might play a role in the development of BD. However, gaps in knowledge persist regarding the expression patterns of RASGRP1 in BD cell lines and its response to mood-related stimuli, such as the stress hormone cortisol since stress is known to trigger episodes in BD patients. To address the gap, the proposed research aims to shed light on the connection between RASGRP1 and BD using Lymphoblastoid cell lines (LCLs) derived from BD and healthy individuals. The first phase of the research seeks to compare RASGRP1 expression patterns between BD I, BD II, and healthy control LCLs using quantitative polymerase chain reaction (qPCR) techniques. Additionally, I will examine the effect of cortisol on RASGRP1 expression in the three cell line groups by dividing cultures into cortisol-treated and control subgroups and measuring subsequent changes in RASGRP1 expression. Investigating RASGRP1 expression and its response to stress hormones, may provide valuable insights into the molecular mechanisms influencing BD development. Ultimately, such knowledge will contribute to improved diagnostic and treatment strategies for this complex psychiatric disorder.